Coding
Part:BBa_K197020:Design
Designed by: Gabriela Guzman Lopez Aguado Group: iGEM09_Berkeley_Wetlab (2009-10-21)
strep
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 22
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 22
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 22
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 22
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This part is in BglBricks Standard. It is flanked by BamHI and BglII sites instead of XbaI and SpeI. More information about the BglBricks Standard is available at:
[http://openwetware.org/wiki/Template:AndersonLab:BBb_Standard BglBricks Standard Description Page]
Source
PCR ca998/ca1363R on pBca9145-Bca9494 (1539 bp, EcoRI/BamHI) Sub into pBca9495CA-Bca1144#5 (EcoRI/BamHI, 3039+910, L) Product is pBca9495CA-Bca1363 {Pbad.rbs.pp.ST}
ca998 Forward Sequencing of pSB1A2/pSB1A3 gtatcacgaggcagaatttcag ca1363R Reverse StrepTag ccataGGATCCctgcccagactgCCCTCCCTC